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KMID : 0613820070170101441
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Journal of Life Science
2007 Volume.17 No. 10 p.1441 ~ p.1446
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Establishment of Genetic Characteristics and Individual Identification System Using Microsatellite Loci in Jeju Native Horse
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Cho Byung-Wook
Jung Ji-Hye
Kim Sang-Wook
Kim Heui-Soo
Lee Hak-Kyo
Cho Gil-Jae
Song Ki-Duk
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Abstract
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This study was conducted to establish the individual identification system and to estimate the genetic characteristic of Jeju native horse (JNH) using 13 microsatellite markers located on different horse autosomes. The markers were genotyped on 191 animals from five horse breeds including Jeju native horse (JNH). In total, 138 alleles were detected from the genotypes of 13 microsatellite markers. The average heterozygosities ranged from 0.317 to 0.902 and the polymorphic information content (PIC) ranged from 0.498 to 0.799 in JNH. We found that there were significant differences in allele frequencies in JNH when compared with other horse breeds. In ATH4 marker, there were specific allele frequence pattern that some of allele only found in JNH, Mongolian horse (MONG) and Jeju racing horse (JRH). The calculated cumulative power of discrimination (CPD) was 99.9% when nine microsatellite loci were used for analysis in the individual identification system. Also, the matching probability that two unrelated animals would show the same genotypes, was estimated to be 0.60×10-10. Therefore, in the nine markers used in this study can be used for individual identification in the Jeju native horse population.
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KEYWORD
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Individual identification, genetic characteristic, polymorphic information content, Jeju native horse
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